|Subject||Tissue Culture for Disease Elimination|
Rapidly growing shoots with terminal buds are preferred sources of plant tissue. The shoot tips are cleaned and surface sterilized to prepare for the aseptic excision under a transfer hood. The youngest cells (meristem) are removed and placed into test tubes with agar media containing precise levels of salts and hormones. Incubation in a growth chamber under controlled conditions until roots and shoots have developed is essential. After 3 to 9 weeks, the plants may be ready to be transplanted into soil and introduced to greenhouse conditions. After further growth and acclimation, they may be planted outdoors.
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Golino, D.A., S.T. Sim, J. Bereczky, and A. Rowhani, 2000. The
Sim, Susan T. 2010. Micro- vs. Macroshoot Tip Tissue Culture Therapy for Disease Elimination in Grapevines (pdf). FPS Grape Program Newsletter, Foundation Plant Services, University of California, Davis, California. October 2010:12-15.
Sim, Susan T. 2006. Virus Elimination from Grape Selections Using Tissue Culture (pdf). FPS Grape Program Newsletter, Foundation Plant Services, University of California, Davis, California. pp30-31.
Sim, Susan T. Grapevine Shoot Tip Culture (pdf). Powerpoint presentation.